A guide RNA repeat checkpoint steers CRISPR-Cas9 catalysis
Ramadevi Chilamkurthy, Sruthi Sudhakar, Adrian A. Pater, Michael S. Bosmeny, Francis Stabile, Adam Katolik, S. Harikrishna, Rolf Turk, Masad J. Damha, Sabrina Leslie, Sergey Korolev, P.I. Pradeepkumar, Keith T. Gagnon
The paper identifies a guide RNA repeat checkpoint that regulates CRISPR-Cas9 catalysis and proposes guide repeat-truncated sgRNAs to improve editing specificity.
bioRxiv · Dec 2, 2025 · PreprintBiochemistry