Rolf Turk

UNCOVERseq is a workflow for sensitive off-target nomination in CRISPR-Cas editing, demonstrating superior performance across multiple modalities and systems.

PASTA quantifies chromosomal translocations from amplicon sequencing with high accuracy and sensitivity, outperforming existing methods in detecting rare rearrangements.

This paper develops gene-edited tacrolimus-resistant Treg cells (FKBP12KO-Treg) for clinical use in transplant recipients, showing they maintain immunoregulatory function while resisting tacrolimus.

The paper identifies a guide RNA repeat checkpoint that regulates CRISPR-Cas9 catalysis and proposes guide repeat-truncated sgRNAs to improve editing specificity.

Empirical ssODN design rules for Cas9, Cas9 D10A and Cas12a HDR, plus end-modified donors and an NHEJ-inhibiting small molecule, raise HDR up to 10-fold.

This study uses gene editing in hematopoietic stem cells to treat Pyruvate Kinase Deficiency Hemolytic Anemia, restoring RPK function and ATP levels in erythroid cells.

CRISPAltRations is a cloud-based tool that improves the analysis of CRISPR-induced DNA repair events by outperforming existing software in annotating indels and HDR events.

This study shows dystroglycan is critical for maintaining inner limiting membrane integrity, which coordinates retinal development and neuronal migration.