Rapid Antibody Fragment Production and Binding Analysis Using Cell-Free Protein Synthesis Combined with Fluorescence Correlation Spectroscopy
Shakiba Nikfarjam, Chao Liu, Emma J. Laurence, Adam N. Laurence, Jennifer L. Chlebek, Dante P. Ricci, Steven A. Hoang-Phou, Ted A. Laurence, Matthew A. Coleman
This paper develops a cell-free protein synthesis method combined with FCS to rapidly produce and characterize antibody fragments, showing improved solubility and binding analysis.
bioRxiv · Aug 5, 2025 · PreprintBiophysics