Steven A. Hoang-Phou

Fluorescence correlation spectroscopy measurements of the chlamydia outer protein B (CopB) made by cell-free protein synthesis

The paper uses fluorescence correlation spectroscopy to analyze YFP-CopB production in cell-free synthesis, measuring its concentration, size, and maturation.

Incorporation of active cell-free expression lysates in chitosan coated alginate microcapsules

This paper demonstrates that chitosan-coated alginate microcapsules can protect cell-free gene expression lysates and proteins from degradation in the GI tract and enable controlled release in simulated intestinal conditions.

Rapid Antibody Fragment Production and Binding Analysis Using Cell-Free Protein Synthesis Combined with Fluorescence Correlation Spectroscopy

This paper develops a cell-free protein synthesis method combined with FCS to rapidly produce and characterize antibody fragments, showing improved solubility and binding analysis.

Real-time affinity measurements of proteins synthesized in cell-free lysate using fluorescence correlation spectroscopy

This paper develops a method to measure protein-protein interactions in real-time using cell-free synthesis and fluorescence correlation spectroscopy, achieving high sensitivity and throughput.

Cell-free screening, production and animal testing of a STI-related chlamydial major outer membrane protein supported in nanolipoproteins.

The paper develops and tests MOMP-NLP complexes as a potential Chlamydia vaccine using cell-free synthesis and preclinical immunization studies.

Evaluation in mice of cell-free produced CT584 as a Chlamydia vaccine antigen

This study evaluates CT584, a Chlamydia antigen produced via cell-free synthesis, as a vaccine in mice, finding it induces antibodies but not protection against infection.

A modular platform for on-demand vaccine self-assembly enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens

This paper introduces AddVax, a method to decorate bacterial outer membrane vesicles with biotinylated antigens to create vaccines, showing strong immune responses in mice.