CRISPR-Cas9 target-strand nicking provides phage resistance by inhibiting replication
Giang T. Nguyen, Akshara Raju, Michael A. Schelling, Dipali G. Sashital
This study shows that Cas9 nicking of the target DNA strand can provide sufficient phage resistance, while non-target nicking does not enhance protection beyond Cas9 binding.
Nucleic Acids Research · Aug 27, 2025Peer-reviewedBiochemistry