Author

Dipali G. Sashital

Iowa State UniversityORCID 0000-0001-7681-6987Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

Click to filter author papers

Biochemistry· 2

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 2 Organism:E. coli· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

Akshara Raju· 1 shared Chaoyou XueiD· 1 shared Elizabeth M. Dougherty· 1 shared Giang T. NguyeniD· 1 shared Jerusha Ravishankar· 1 shared Meriç ÖztürkiD· 1 shared Michael A. SchellingiD· 1 shared Phong Tuan Phan· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

8 top collaborators · 13 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

1 shared paperBiochemistry

Akshara Raju

Mutual co-authors in cluster

Author profile →

2 papers

Recent·Most cited

BiochemistryJournal of Biological ChemistryPeer-reviewedMar 26, 2026

Mismatch type impacts interference and priming activities in the type I-E CRISPR-Cas system

Phong Tuan Phan, Meriç Öztürk, Elizabeth M. Dougherty…·Dipali G. Sashital

This study investigates how crRNA seed sequence mismatches affect interference and priming in the type I-E CRISPR-Cas system, finding that specific mismatches cause significant defects in immune function.

DOI 10.1016/j.jbc.2026.111401

E. coliexperimental assay

𝕏 PostBluesky

BiochemistryNucleic Acids ResearchPeer-reviewedAug 27, 2025

CRISPR-Cas9 target-strand nicking provides phage resistance by inhibiting replication

Giang T. Nguyen, Akshara Raju, Michael A. Schelling, Dipali G. Sashital

This study shows that Cas9 nicking of the target DNA strand can provide sufficient phage resistance, while non-target nicking does not enhance protection beyond Cas9 binding.

4 citations

experimental assayhost-pathogen & immune response

𝕏 PostBluesky