Author

Michael A. Schelling

Iowa State UniversityORCID 0000-0003-1739-503XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:host-pathogen & immune response· 2 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

Akshara Raju· 2 shared Dipali G. SashitaliD· 2 shared Aneisha Sritharan· 1 shared Giang T. NguyeniD· 1 shared Giang T. NguyeniD· 1 shared Kathryn A. BuscheriD· 1 shared

Activity by Year

2024-2025 · peak 1/yr

Co-Author Collaboration Network

6 top collaborators · 12 mutual co-author links

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2 shared papersBiochemistry

Akshara Raju

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2 papers

Recent·Most cited

BiochemistryNucleic Acids ResearchPeer-reviewedAug 27, 2025

CRISPR-Cas9 target-strand nicking provides phage resistance by inhibiting replication

Giang T. Nguyen, Akshara Raju, Michael A. Schelling, Dipali G. Sashital

This study shows that Cas9 nicking of the target DNA strand can provide sufficient phage resistance, while non-target nicking does not enhance protection beyond Cas9 binding.

4 citations

experimental assayhost-pathogen & immune response

𝕏 PostBluesky

BiochemistryNucleic Acids ResearchPeer-reviewedJul 1, 2024

CRISPR-Cas12a exhibits metal-dependent specificity switching

Giang T. Nguyen, Michael A. Schelling, Akshara Raju…·Dipali G. Sashital

This study shows that Cas12a's specificity changes with Mg2+ concentration, affecting cleavage efficiency and phage escape routes in different cellular environments.

54 citationstop 2%

experimental assayhost-pathogen & immune response

𝕏 PostBluesky