Scaling Multiplex qPCR Primer Design to 1000-plex using the Degenerate Incomplete Multiplex Primer List Extension (DIMPLE) Algorithm
Alessandro Pinto, Xue Dong, Wei Wu, Sara J. Johnson, Qin Wen, Cheng Zhang, Jana Havey, Bonnie Wang, Guilin Tang, Aziz Farhat, David Yu Zhang, Ghayas C. Issa, Xi Zhang
The paper introduces DIMPLE, a scalable qPCR primer design algorithm that enables 10,000+ primers in a single reaction, achieving low VAF detection and multiplex applications.
bioRxiv · Apr 21, 2026 · PreprintBioengineering