Dissecting the stability determinants of a challenging de novo protein fold using massively parallel design and experimentation
Tae-Eun Kim, Kotaro Tsuboyama, Scott Houliston, Cydney M. Martell, Claire M. Phoumyvong, Alexander S. Lemak, Hugh K. Haddox, C.H. Arrowsmith, Gabriel J. Rocklin
The paper designs over ten thousand {square}{beta}{beta}{square} proteins, identifies stability determinants, and demonstrates how high-throughput methods can improve protein design success rates.
Proceedings of the National Academy of Sciences · Oct 3, 2022Peer-reviewedSynthetic Biology