DNA Ligases Discriminate Between Natural and Non-Natural Base Pairs
Caitlin Walters-Freke, Shuichi Hoshika, Alexandra Perry, Steven A. Benner, Renwick C. J. Dobson, Zachary D. Tillett, Nigel G. J. Richards, Adele Williamson
The paper investigates how DNA ligases handle non-canonical base pairs in hachimoji DNA, finding that consecutive non-canonical bases at the 3-end reduce ligation efficiency.
bioRxiv · Jul 1, 2026 · PreprintBiochemistry