CaBLAM! A high-contrast bioluminescent Ca2+ indicator derived from an engineered Oplophorus gracilirostris luciferase
Gerard G. Lambert, Emmanuel L. Crespo, Jeremy Murphy, Kevin L. Turner, Emily Gershowitz, Michaela Cunningham, Daniela Boassa, Selena Luong, Dmitrijs Celinskis, Justine J. Allen, Stephanie Venn, Yunlu Zhu, Mürsel Karadas, Jiakun Chen, Roberta Marisca, , Nathan C. Shaner
This paper introduces CaBLAM, a bioluminescent Ca2+ indicator with improved dynamic range and Ca2+ affinity, enabling high-resolution imaging of cellular activity in cultured neurons and in vivo.
Nature Methods · Dec 2, 2025Peer-reviewedNeuroscience