Efficient installation of heterozygous mutations in human pluripotent stem cells using prime editing
Annabelle Suter, Alison N. Graham, Jia Yi Kuah, Jason Crisologo, Chathuni Gunatilake, Koula Sourris, Michael See, Fernando Jaime Rossello, Mirana Ramialison, Katerina Vlahos, Sara E. Howden
This study demonstrates the use of prime editing to efficiently introduce heterozygous mutations in human pluripotent stem cells with high knock-in efficiency and without purification or selection steps.
The CRISPR Journal · Sep 24, 2025Peer-reviewedCell Biology