Identifying a cooperative catalytic network for efficient esterase catalysis
Sharad Sarak, Hong Yang, Colin T. Pierce, Panhavuth Tan, Allison Cafferty, Anne Dao, Dana Junaidi, Ke Shi, Robert L. Evans, Romas J. Kazlauskas
The paper redesigns an ancestral enzyme's active site to enhance esterase activity through cooperative residue substitutions and structural changes.
bioRxiv · May 31, 2026 · PreprintBiochemistry