Author

Sharad Sarak

University of MinnesotaORCID 0000-0002-6087-1292Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 2

Signature Assays, Organisms & Methods

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Assay:protein structure· 2 Approach:experimental assay· 1 Organism:plant (other)· 1 Research Focus:protein folding & dynamics· 1 Research Focus:protein structure prediction· 1 Approach:statistical model· 1

Frequent Co-Authors

Colin T. PierceiD· 2 shared Ke ShiiD· 2 shared Panhavuth TaniD· 2 shared Robert L. EvansiD· 2 shared Romas J. KazlauskasiD· 2 shared Alana H. NguyeniD· 1 shared Allison Cafferty· 1 shared Anne Dao· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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2 shared papersBiochemistry

Colin T. PierceORCID

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2 papers

Recent·Most cited

BiochemistrybioRxivPreprintMay 31, 2026

Identifying a cooperative catalytic network for efficient esterase catalysis

Sharad Sarak, Hong Yang, Colin T. Pierce…·Romas J. Kazlauskas

The paper redesigns an ancestral enzyme's active site to enhance esterase activity through cooperative residue substitutions and structural changes.

DOI 10.64898/2026.05.29.728779

protein structurestatistical model

𝕏 PostBluesky

BiochemistrybioRxivPreprintMar 30, 2025

Crystal structures of forty- and seventy-one-substitution variants of hydroxynitrile lyase from rubber tree

Colin T. Pierce, Panhavuth Tan, Lauren R. Greenberg…·Romas J. Kazlauskas

The paper describes the creation and structural analysis of HbHNL variants with 40 and 71 mutations to resemble SABP2, showing increased structural similarity to SABP2 with more mutations.

DOI 10.1101/2025.03.30.646168

plant (other)protein structure

𝕏 PostBluesky