Deep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite de novo Protein Sequencing by Mass Spectrometry
Tatiana M. Shamorkina, Laura Pérez Pañeda, Tereza Kadavá, Douwe Schulte, Patrick A. Pribil, Sibylle Heidelberger, Allison Michele Narlock-Brand, Steven M. Yannone, Joost Snijder, Albert J. R. Heck
This paper introduces HTA-proteases for de novo antibody sequencing, achieving higher peptide read coverage and accuracy compared to trypsin or chymotrypsin.
bioRxiv · May 26, 2025 · PreprintBiochemistry