Albert J. R. Heck

The paper examines IgG4 antibody repertoire stability over time in health and autoimmune diseases, finding that most clones remain stable despite treatment.

The paper identifies pregnancy-specific and fetal-specific plasma proteomic and N-glycoproteomic signatures by comparing maternal, cord blood, and non-pregnant control samples.

The paper investigates how metabolon clustering enhances coenzyme Q biosynthesis through substrate channeling using simulations and experiments.

This study shows that MIB protein binds all human antibody isotypes, reduces Fab flexibility, and displaces antigens in immune complexes without affecting Fc interactions.

This study identifies immunoglobulin sub-class levels as key factors in inter-donor plasma variability using longitudinal proteomic data from two labs.

The paper introduces AbDirect, a proteomics-based method for antibody discovery that identifies potent neutralizing antibodies missed by single-cell BCR sequencing in COVID-19 plasma.

The paper identifies structurally promiscuous IgA1 clones in human serum that exist as both monomers and J-chain-coupled dimers, challenging the view that these forms are clonally distinct.

This study shows that some anti-topoisomerase 1 autoantibodies (ATAs) in systemic sclerosis (SSc) bind more strongly to TOP1-DNA cleavage complexes, correlating with disease severity and immune activation.

This study identifies cyclin-dependent kinases as key drivers of Ebola virus replication and transcription through proteomic analysis of host cell remodeling.

This paper introduces MBG, a method to expand glycopeptide identification in glycoproteomics by leveraging MS1 signals from displaced glycopeptides, improving detection of low-abundant and modified glycans.

The paper identifies foamy microglia as contributors to disease progression in secondary progressive MS, linking oxylipins to lesion expansion and proposing monoacylglycerol lipase as a therapeutic target.

This study identifies a novel link between ATP synthase and the TCA cycle using crosslinking mass spectrometry, showing how ATP synthase interacts with TCA cycle enzymes and adapts under mitochondrial dysfunction.

The paper introduces a universal tool for annotating peptide spectra in proteomics, supporting various fragmentation methods and modifications.

This study shows that using EThcD on a new mass spectrometer improves the identification and sequence coverage of HLA class I immunopeptidomes by filling gaps in fragmentation data.

This paper develops a protocol to accurately measure the mass of large DNA plasmids using mass photometry by converting dsDNA to ssDNA-like particles, achieving mass accuracy within 1-3%.

This paper introduces HTA-proteases for de novo antibody sequencing, achieving higher peptide read coverage and accuracy compared to trypsin or chymotrypsin.

This study generates bispecific antibodies targeting two distinct HCV E1E2 epitopes, showing enhanced neutralization breadth and potency compared to monospecific antibodies.

The paper introduces an LC-MS method to analyze IgG4 repertoires with clonal resolution, revealing high diversity and persistence of IgG4 molecules in serum over time.

The paper identifies oxygen-induced tubular nanocompartments in Pyrococcus furiosus using proteomics and cryo-EM, revealing their structure and role in ROS protection.

The paper evaluates the Orbitrap Astral mass spectrometer for plasma glycoproteome analysis, introducing nGlycoDIA to achieve high sensitivity and throughput.

This study shows that single mutations during early oncogenesis can upregulate BTN2A1, enabling Vγ9Vδ2 TCR binding, but full activation requires subsequent phosphorylation of BTN3A1.

This study identifies F-actin as part of the manchette and reveals two distinct transport mechanisms for intra-manchette transport during spermiogenesis.

This study uses single-molecule interferometric scattering microscopy to analyze AAV capsid stability and genome release under thermal stress, revealing AAV-specific uncoating behaviors.

This study tracks longitudinal changes in the plasma proteome and complexome of kidney failure patients before and after transplantation, revealing acute phase protein dynamics and macromolecular complex formation during inflammation.

The paper identifies CD5L as a component of circulatory IgM, showing it forms a complex with J-chain-containing pentamers and affects IgM receptor binding.

This study shows that mice with reduced respirasomes maintain normal bioenergetics and physiology, suggesting respirasomes are not essential for basic function but may have other roles.

The paper quantifies kinase activity dynamics in response to FGF ligands and uses dynamic modeling to reveal pathway regulation and novel kinase involvement.

The paper identifies a previously overlooked complex between AIFM1 and cytochrome c oxidase using mass spectrometry and proteomic approaches, revealing structural insights and potential roles in OXPHOS and cell death.

This study shows mitochondrial CaMKII activation leads to metabolic reprogramming, energetic insufficiency, and dilated cardiomyopathy, with potential therapeutic targets identified.