Distinct 5' and 3' Coverage Biases Shape Transcriptome Interpretation in Nanopore Direct RNA versus PCR-cDNA Sequencing
Rebecca E. Lane, Eleanor Calcutt, Anandagopal Srinivasan, Vicki Gamble, Udo C. T. Oppermann, Jianfeng Sun, Adam P. Cribbs
This study compares nanopore direct RNA and PCR-cDNA sequencing protocols, showing distinct 5' and 3' coverage biases affecting transcript-level analysis but similar gene-level accuracy.
BMC Genomics · Jul 15, 2026Peer-reviewedCell Biology