Vicki Gamble

Distinct 5' and 3' Coverage Biases Shape Transcriptome Interpretation in Nanopore Direct RNA versus PCR-cDNA Sequencing

This study compares nanopore direct RNA and PCR-cDNA sequencing protocols, showing distinct 5' and 3' coverage biases affecting transcript-level analysis but similar gene-level accuracy.

A systems-based approach to uterine fibroids identifies differential splicing associated with abnormal uterine bleeding

The paper integrates multi-omics data from uterine fibroids, myometrium, and endometrium to identify differential splicing and molecular pathways associated with abnormal uterine bleeding.

ATF4-Mediated Metabolic Stress Response as a Therapeutic Vulnerability in Chordoma

This study identifies ATF4-mediated metabolic stress response as a therapeutic target in chordoma, showing that EPRS inhibitors reduce cell viability and inhibit tumor growth in vivo.

Anchor-Enhanced Bead Design for Reduced Oligonucleotide Synthesis Errors in Single-cell sequencing

The paper introduces an anchor-enhanced bead design to reduce oligonucleotide synthesis errors in single-cell sequencing, improving UMI recovery and feature detection.

Correcting PCR amplification errors in unique molecular identifiers to generate absolute numbers of sequencing molecules.

The paper demonstrates that PCR errors significantly affect UMI-based absolute counting in sequencing data and proposes a solution using homotrimeric nucleotide blocks to correct these errors.