Engineering a Glucose-Inducible Whole-Cell Biosensor via CRISPRi-Based Promoter Reprogramming
Mani Gupta, Anshuman Das, Satyadip Paul, Supratim Datta
This paper develops a CRISPRi-based glucose-inducible biosensor in E. coli that converts native glucose repression into a tunable fluorescent output, with high specificity and sensitivity for glucose monitoring.
bioRxiv · Apr 11, 2026 · PreprintBioengineering