Fluorescence Characterization of Extracellular Vesicles using Single-Molecule Confocal Microscopy
Tianxiao Zhao, Noelia Pelegrina‐Hidalgo, Daniel C. Edwards, Krzysztof M. Bąk, Utsa Karmakar, Anuruddika J. Fernando, Marc Vendrell, Adriano Giorgio Rossi, Scott L. Cockroft, Tilo Kunath, Rebecca S. Saleeb, Mathew H. Horrocks
This study develops a method to detect and quantify extracellular vesicles using fluorescent antibodies and single-molecule confocal microscopy, achieving femtomolar sensitivity without purification.
Small Methods · Aug 17, 2025Peer-reviewedBiophysics