Mathew H. Horrocks

The paper introduces NanoSYNMAP to map synaptic nanoarchitecture across the mouse lifespan, revealing how molecular proximity shapes synapse diversity.

This paper introduces H-MIP, a high-throughput platform for mitochondrial morphology analysis, and applies it to study mitochondrial changes in vascular calcification.

The paper identifies microglial activation and alpha-synuclein oligomers as drivers of early neuroinflammation in Parkinson's disease using transcriptomic and aggregate profiling in early-stage brain samples.

This study develops a method to detect and quantify extracellular vesicles using fluorescent antibodies and single-molecule confocal microscopy, achieving femtomolar sensitivity without purification.

This paper shows that ME0054 disrupts AdhE spirosomes, enhancing ethanol-to-acetyl-CoA conversion and altering T3SS expression in EHEC.

This study uses RNA aptamers to detect pathological FUS in ALS, showing improved specificity and sensitivity compared to antibodies.

The paper introduces VP-CLEM-Kit, a cost-effective SR-vCLEM pipeline for visual proteomics, achieving 5-fold resolution improvement at 7-fold lower cost.

This study validates secondary nanobodies for multiplexed immunoassays and provides optimized protocols for their use.

This study adapts the LIVE-PAINT method for live mammalian cells, demonstrating super-resolution imaging of two proteins in real time.

This study uses 3D super-resolution microscopy to analyze PSD95 nanoarchitecture in the mouse brain, revealing diffuse supercomplexes outside synapses and distinct subtypes within synapses.

The paper investigates the sequential replacement of PSD95 subunits in synaptic supercomplexes, finding that this process is slowest in the cortex where PSD95 has the longest lifetime.

This study shows GLP-1R agonism improves Parkinson's disease by restoring neuronal insulin signaling and reducing glial inflammation, with clinical benefits in patients with higher baseline MAPK expression.

This study uses an RNA aptamer to detect TDP-43 aggregation and shows that nuclear TDP-43 pathology and STMN-2 cryptic splicing occur before clinical symptoms in ALS.

This study uses super-resolution microscopy to characterize phosphorylated TDP-43 expression in spinal cord synapses of ALS mice and healthy controls.

This paper introduces a method to specifically detect disease-associated protein aggregates using two-color coincidence single-molecule pull-down, demonstrating its effectiveness with alpha-synuclein in human biofluids.

The paper introduces direct-LIVE-PAINT, a method for nanoscopic imaging of protein structures in live cells, demonstrating its application to actin with sub-diffraction resolution.

This study uses hiPSCs with SNCA mutations to identify early molecular events in synucleinopathy, showing protein aggregation precedes functional impairments in midbrain dopaminergic neurons.

The paper tracks α-synuclein conformational changes in neurons and shows mitochondrial involvement in oligomerization and toxicity.

LIVE-PAINT enables super-resolution imaging in live cells by using reversible peptide-protein interactions instead of direct fluorescent protein fusion.