Distal mutations in a designed retro-aldolase alter loop dynamics to shift and accelerate the rate-limiting step
Serena E. Hunt, Cindy Klaus, Aqza E. John, Niayesh Zarifi, A J Sanchez-Jauregui Martinez, Ferran Feixas, Marc Garcia‐Borràs, Michael C. Thompson, Roberto A. Chica
The paper investigates how distal mutations in a retro-aldolase affect loop dynamics and catalytic efficiency, showing an epistatic effect when combined with active-site mutations.
Journal of the American Chemical Society · Aug 13, 2025Peer-reviewedBiochemistry