Niayesh Zarifi

The paper designs de novo TIM barrels with structural lids to create active sites for enzymatic function, achieving high catalytic efficiency.

This study shows that distal mutations improve enzyme catalysis by enhancing substrate binding and product release, working with active-site mutations to increase overall activity.

The paper investigates how distal mutations in a retro-aldolase affect loop dynamics and catalytic efficiency, showing an epistatic effect when combined with active-site mutations.