Highly Efficient Multiplexed Genome Engineering and Clone Selection to Enable Next Generation Induced Pluripotent Stem Cell (iPSC)-based Cell Therapies
Kaivalya Molugu, Ruba Halaoui, Mohammed Zeaiter, Anne M. Bara, Matthew H. Bakalar, Bruna Castro de Oliveira, Connor McGinnis, Jie Wang, Marie-Joe Kimaz, Lauren Herchenroder, Arun Bhattathiri, Alexander Allen, Minggang Fang, Davood Norouzi, Didac Santesmasses, , Chong T. Luo
This paper introduces a novel genome engineering method for iPSCs that enables efficient multiplex gene editing and clone selection, improving the precision and speed of iPSC-based therapies.
bioRxiv · Dec 25, 2025 · PreprintGenomics