Membrane proteins retain native architecture through native ESI and soft-landing
Jingjin Fan, Clare De'Ath, L. Eriksson, Carl von Hallerstein, Louise Jeanette Pauline Persson, Abraham Olusegun Oluwole, Noor Naseeb, Aziz Qureshi, Susanne M. Mesoy, Laurence T. Seeley, Simon B. Knoblauch, Neha V. Kalmankar, Erik G. Marklund, Tim K. Esser, Carol V. Robinson, , Stephan Rauschenbach
This study shows that native electrospray ion beam deposition preserves membrane protein structure, enabling high-resolution cryo-EM analysis of complexes like AmtB.
bioRxiv · Jan 13, 2026 · PreprintBiophysics