Tim K. Esser

This paper demonstrates a method to rehydrate proteins after soft-landing for cryo-EM by flash melting, enabling native configurations and improving sample preparation.

This study shows that native electrospray ion beam deposition preserves membrane protein structure, enabling high-resolution cryo-EM analysis of complexes like AmtB.

This paper introduces native ES-IBD for preparing high-purity, ice-free cryo-EM samples, showing structural integrity but limitations in 2D classes and 3D EM density maps.