Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos
J. R. Owen, S. L. Hennig, B. R. McNabb, J. C. Lin, A. E. Young, J. D. Murray, P. J. Ross, A. L. Van Eenennaam
This study compares gene knock-in efficiency in bovine zygotes using homology-directed repair (HDR) and homology-mediated end joining (HMEJ) templates, finding HMEJ achieves higher knock-in rates and lower mosaicism.
bioRxiv · Jun 9, 2020Bioengineering