A Method for Rapid Flow-cytometric Isolation of Endothelial Nuclei and RNA from Archived Frozen Brain Tissue
A. L. Kimble, J. Silva, M. Murphy, J. A. Hensel, S.-A. E. Nicholas, E. R. Jellison, B. Reese, P. A. Murphy
This paper develops a method to isolate endothelial nuclei and RNA from archived frozen brain tissues, showing it reliably extracts endothelial RNA and correlates with whole-cell expression.
bioRxiv · May 12, 2021Cell Biology