Can we put Humpty Dumpty back together again? What does protein quantification mean in bottom-up proteomics?
D. L. Plubell, L. Käll, B.-J. Webb-Robertson, L. Bramer, A. Ives, N. L. Kelleher, L. M. Smith, T. J. Montine, C. C. Wu, M. J. MacCoss
The paper questions the assumption that all peptides from a protein coding gene represent the same protein quantity in bottom-up proteomics, highlighting the need for better methods to account for proteomic complexity.
bioRxiv · Jan 25, 2021 · PreprintBiochemistry