N. L. Kelleher

Terpenoid balance in Aspergillus nidulans unveiled by heterologous squalene synthase expression

The paper shows that expressing a heterologous squalene synthase from Pseudogymnoascus destructans in Aspergillus nidulans alters terpene biosynthesis, increasing squalene and new nidulenes while reducing austinol.

Epi-microRNA mediated metabolic reprogramming ensures affinity maturation

This study identifies miR-155's role in metabolic reprogramming of GC-B cells to support affinity maturation under hypoxic conditions.

Top-down Proteomics of 10,000 Single Brain Cells

The paper introduces scPiMS, a single-cell proteomics method, and identifies proteoforms from 10,836 rat hippocampal cells, classifying them into neuron, astrocyte, and microglia types.

Direct Imaging and Identification of Proteoforms up to 70 kDa from Human Tissue

This paper introduces PiMS, a method that detects proteoforms up to 70 kDa in human tissue with high spatial resolution, identifying key metabolic enzymes in kidney tissue.

Elevated N-glycosylation of immunoglobulin G variable regions in myasthenia gravis highlights a commonality across autoantibody-associated diseases

This study identifies elevated N-glycosylation in IgG variable regions in myasthenia gravis patients and shows it is not required for autoantigen binding.

Can we put Humpty Dumpty back together again? What does protein quantification mean in bottom-up proteomics?

The paper questions the assumption that all peptides from a protein coding gene represent the same protein quantity in bottom-up proteomics, highlighting the need for better methods to account for proteomic complexity.

Separation and Characterization of Endogenous Nucleosomes by Native Capillary Zone Electrophoresis - Top-Down Mass Spectrometry (nCZE-TDMS)

The paper introduces nCZE-TDMS, a platform for separating and characterizing nucleosomes, histone subunits, and PTMs, and applies it to study histone modifications in cell lines with altered NSD2/MMSET expression.

Standard Procedures for Native CZE-MS of Proteins and Protein Complexes up to 800 kDa

The paper provides standard operating procedures for native capillary electrophoresis mass spectrometry (nCZE-MS) to improve reproducibility and data quality for large proteins and complexes.

An Interpreted Atlas of Biosynthetic Gene Clusters from 1000 Fungal Genomes

The paper systematically analyzes biosynthetic gene clusters from 1000 fungal genomes to create an interpreted atlas of fungal natural products and their chemical space.

Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS

The paper introduces Nuc-MS, a method to analyze protein composition of whole nucleosomes, revealing specific histone variants and modifications associated with oncogenic states.

Multiplexed Single Ion Mass Spectrometry Improves Measurement of Proteoforms and Their Complexes

This paper introduces a new Orbitrap-based single ion analysis method that improves the measurement of proteoforms and their complexes by directly determining ion charge.

Direct Measurement of Light and Heavy Antibody Chains Using Differential Ion Mobility Spectrometry and Middle-Down Mass Spectrometry

This paper introduces a rapid FAIMS tandem MS method for characterizing monoclonal antibody light and heavy chains, reducing analysis time by 40-60% compared to LC-MS/MS.

USP7 cooperates with NOTCH1 to drive the oncogenic transcriptionalprogram in T cell leukemia

This study identifies USP7 as a key driver of T-cell leukemia by stabilizing NOTCH1 and JMJD3, showing USP7 inhibition blocks leukemia growth and suggests it as a therapeutic target.