Rationally designed mariner vectors to allow functional genomic analysis of Actinobacillus pleuropneumoniae and other bacteria by transposon-directed insertion-site sequencing (TraDIS)
J. T. Bosse, Y. Li, L. G. Leanse, L. Zhou, R. R. Chaudhuri, S. E. Peters, J. Wang, G. A. Maglennon, M. T. G. Holden, D. J. Maskell, A. W. Tucker, B. W. Wren, A. N. Rycroft, P. R. Langford, BRaDP1T consortium
This study develops two novel mariner vectors for TraDIS to identify conditionally essential genes in A. pleuropneumoniae and P. multocida, identifying 8 and 15 genes required for anaerobic growth.
bioRxiv · Oct 3, 2018 · PreprintMicrobiology