Optimized genome-wide CRISPR screening enables rapid engineering of growth-based phenotypes in Yarrowia lipolytica
Nicholas R. Robertson, Varun Trivedi, Brian Lupish, Adithya Ramesh, Yuna Aguilar, Anthony Arteaga, Alexander Nguyen, Sang Cheon Lee, Chase Lenert-Mondou, Marcus Harland‐Dunaway, Robert E. Jinkerson, Ian R. Wheeldon
The paper designs a high-activity CRISPR sgRNA library for Yarrowia lipolytica and uses it to identify genes involved in acetate and hydrocarbon tolerance.
bioRxiv · Jun 20, 2024 · PreprintSynthetic Biology