Varun Trivedi

Optimized genome-wide CRISPR screening enables rapid engineering of growth-based phenotypes in Yarrowia lipolytica

The paper designs a high-activity CRISPR sgRNA library for Yarrowia lipolytica and uses it to identify genes involved in acetate and hydrocarbon tolerance.

Functional genomic screening in Komagataella phaffii enabled by high-activity CRISPR-Cas9 library

The paper develops a high-activity CRISPR-Cas9 library for Komagataella phaffii and uses it to identify essential genes related to protein production, secretion, and glycosylation.

acCRISPR: An activity-correction method for improving the accuracy of CRISPR screens

acCRISPR improves CRISPR screen accuracy by correcting sgRNA activity, identifying essential genes in yeast under various conditions.

Population genomics-guided engineering of phenazine biosynthesis in Pseudomonas chlororaphis

This study uses population genomics to identify genetic targets for improving phenazine biosynthesis in Pseudomonas chlororaphis through genome-wide association analysis and experimental validation.