Impact of RNA Extraction and Target Capture Methods on RNA Sequencing Using Formalin-Fixed, Paraffin Embedded Tissues
Christopher A. Hilker, Aditya Vijay Bhagwate, Jin Sung Jang, Jeffrey G. Meyer, Asha Nair, Jaime I Davila, Amber M. McDonald, Jennifer L. Winters, Rebecca N. Wehrs, Rory A. Jackson, Joshua A. Gorman, Mine S. Cicek, André M. Oliveira, E. Aubrey Thompson, Bruce W. Eckloff, , Jin Jen
This study evaluates RNA extraction and capture methods for RNA-seq from FFPE tissues, finding high correlation in gene expression profiles with sufficient RNA quality and demonstrating improved fusion detection with TruSeq RNA Access.
bioRxiv · May 31, 2019 · PreprintGenomics