SIBR-Cas enables host-independent and universal CRISPR genome engineering in bacteria
C. Patinios, S. Creutzburg, A. Arifah, B. Perez, C. Ingham, S. Kengen, J. van der Oost, R. Staals
This paper introduces SIBR-Cas, a CRISPR-based genome engineering tool that works universally in bacteria without host factors or exogenous recombinases, enabling gene knockouts in species with poor homologous recombination.
bioRxiv · Apr 26, 2021Bioengineering