BioengineeringbioRxiv
SIBR-Cas enables host-independent and universal CRISPR genome engineering in bacteria
C. Patinios, S. Creutzburg, A. Arifah…·R. Staals
This paper introduces SIBR-Cas, a CRISPR-based genome engineering tool that works universally in bacteria without host factors or exogenous recombinases, enabling gene knockouts in species with poor homologous recombination.
DOI 10.1093/nar/gkab893