Single-Cell Isolation and Patient-Derived Organoid Generation Using the Pala™ Single Cell Dispenser with Cancer Cell Lines Spiked into Blood as a Circulating Tumour Cell Model: A Platform for Precision Oncology and Drug Discovery
Shambhavi Krishna, Fatma Yaren Giray, Musha Yang, Karolina Stirblyte, Steven G. Gray, Feras Abu Saadeh, Marie Reidy, Cara Martin, Sharon A. O'Toole, Doug A. Brooks, Stavros Selemidis, Derek G. Doherty, Elena Matsa, John J. O`Leary, Steven Johnstone, Bashir M. Mohamed
This paper validates the Pala™ Single Cell Dispenser for isolating cancer cells and generating patient-derived organoids, showing higher efficiency and viability than FACS.
bioRxiv · Jun 16, 2026 · PreprintCancer Biology