Bispecific Coiled-Coil Reporters Enable Rapid, Multiplexed Detection of Mycoplasma via Toehold Switch Sensors
Yudan Li, Nery R. Arevalos, Amit Eshed, Shannon M. Newell, Carlye Frisch, Nin Rebecca Kang, Xiao Wang, David A. Brafman, Alexander A. Green
This paper introduces BINOCULAR, a system for rapid, multiplexed detection of Mycoplasma using toehold switch sensors, achieving sub-5-minute readout times and 1-copy/µL sensitivity.
ACS Synthetic Biology · Oct 5, 2026Peer-reviewedSynthetic Biology