Caroline E. Copeland

Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expression

This paper optimizes low-cost reagent formulations for cell-free gene expression, achieving high yields and cost reductions across multiple scales and protein types.

MEMO: A Micro Memo Sensor Detecting microRNA-RISC using an Accurate Cell-Free Expression Platform

The paper introduces a cell-free biosensor for detecting microRNA-RISC interactions with high sensitivity and specificity.

Expanding the Cell-Free Reporter Protein Toolbox by Employing a Split mNeonGreen System to Reduce Protein Synthesis Workload

This study improves cell-free biosensor performance by using split mNeonGreen proteins with SynZip linkers, enhancing fluorescence and reducing energy use.

Suitability Evaluation of Toehold Switch and EXPAR for Cell-Free MicroRNA Biosensor Development

This paper evaluates the suitability of toehold switch and EXPAR for developing a cell-free miRNA biosensor, finding that EXPAR enhances detection sensitivity and accuracy.

Characterizing a New Fluorescent Protein for Low Limit of Detection Sensing in the Cell-Free System

This study characterizes a new fluorescent protein, mNeonGreen, to improve the low limit of detection in cell-free biosensors by optimizing DNA sequence and reaction conditions.