James R. Swartz

Hydrogen-Driven Cell-Free Cofactor Regeneration Enables Stoichiometric Bioconversion of Pyruvate to Lactate

This study demonstrates hydrogen-driven cofactor regeneration for efficient pyruvate-to-lactate conversion using engineered enzymes.

Genome mining reveals a sporulation associated protein with ferredoxin NADP+ reductase activity in Clostridium pasteurianum: structural and kinetic characterization

The paper identifies a novel ferredoxin-NADP+ reductase in C. pasteurianum, characterizes its structure and function, and reveals its role in redox regulation during sporulation.

Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expression

This paper optimizes low-cost reagent formulations for cell-free gene expression, achieving high yields and cost reductions across multiple scales and protein types.

On-demand insulin manufacturing using cell-free systems with an "on-column" conversion approach

This paper develops a cell-free system for rapid insulin production using on-column conversion, improving accessibility and reducing supply chain strain.

Scalable cell-free production of active T7 RNA polymerase

This paper demonstrates scalable cell-free production of active T7 RNA polymerase with high purity and activity, suitable for decentralized biotherapeutic manufacturing.

Secretion and Periplasmic Activation of a Potent Endonuclease in E. coli

This study optimizes the recombinant production of Sm Endonuclease in E. coli by identifying effective signal sequences and conditions for periplasmic translocation and activity accumulation.

Toward a Genome Scale Sequence Specific Dynamic Model of Cell-Free Protein Synthesis in Escherichia coli

This study develops a dynamic model of E. coli cell-free protein synthesis, showing that oxidative phosphorylation, glycolysis, and amino acid biosynthesis are key for productivity.

Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis

This paper uses sequence-specific constraint-based modeling to estimate the performance of E. coli cell-free protein synthesis systems using limited parameters.