Michael C. Jewett

The paper discovers and engineers an orthogonal translation system using metagenomic data to expand the genetic code with non-canonical amino acids.

Inverse FoldDir designs protein sequences compatible with a given backbone structure, achieving high accuracy and experimental validation in nanobody redesign.

This paper develops a cell-free biosynthesis system for 1,2,4-butanetriol, achieving high productivity and yield at scale while reducing costs.

The paper identifies a unique structural break in octopus ribosomal RNA that enhances translation fidelity and supports proteomic stability through RNA editing.

The paper uses machine learning to guide cell-free expression of carbonic anhydrases, identifying variants with improved stability under high temperatures.

This paper optimizes low-cost reagent formulations for cell-free gene expression, achieving high yields and cost reductions across multiple scales and protein types.

The paper describes methods to make a membrane-bound glycosyltransferase water-soluble for easier study and application in synthetic glycobiology.

This paper designs soluble, miniaturized MHC proteins that enable stable expression in E. coli and retain peptide and TCR binding specificity.

The paper develops a machine learning-guided workflow to optimize cell-free biosensors for detecting lead in drinking water, achieving sensitivity at the EPA action level and improved selectivity.

This paper presents a cell-free manufacturing platform for producing conjugate vaccines with high glycosylation efficiency and purity, validated through immunogenicity testing in mice.

The paper evaluates cofactor recycling and permeability in bacterial microcompartments using an in vitro assay and kinetic model of the Pdu system, showing benefits for pathway flux.

This paper demonstrates scalable cell-free production of active T7 RNA polymerase with high purity and activity, suitable for decentralized biotherapeutic manufacturing.

This paper develops a semi-automated method for manufacturing cell-free biosensors using liquid handling robotics, demonstrating comparable performance to manual methods.

The paper develops a machine learning-guided cell-free platform to accelerate enzyme engineering by mapping sequence-function relationships and optimizing enzymes for multiple chemical reactions.

The paper develops and optimizes a one-pot NASBA-Cas13a assay for rapid, sensitive detection of SARS-CoV-2 RNA without specialized equipment.

The paper identifies a single-subunit oligosaccharyltransferase from Desulfovibrio marinus that enables glycosylation of full-length IgG antibodies in E. coli.

This study presents a cell-free biocatalytic system for converting one-carbon substrates into acetyl-CoA using a synthetic pathway called ReForm.

The paper develops a chloroplast-based cell-free gene expression platform across multiple plant species to accelerate synthetic biology research and gene expression studies.

The paper identifies alternate conformational pathways in ribosome translocation by combining directed evolution and molecular dynamics simulations, revealing diverse kinetic and structural dynamics.

This paper develops a cell-free platform combining CFPS and AlphaLISA to rapidly study and engineer PTM installing proteins for therapeutic applications.

The paper validates a cell-free protein synthesis platform in microgravity by testing biosensors for small molecules and RNA sequences aboard the International Space Station.

The paper develops a chloroplast cell-free system to measure ribosome binding site strengths by testing a library of RBS variants and ranking them based on gene expression.

The paper introduces a fluorescent minihelix assay for quantifying translation in cell-free systems using Flash dye and demonstrates its utility for real-time and terminal detection of protein expression.

The paper develops four cell-free biosensing modules for synthetic biology education that enable students to explore transcriptional regulation and environmental monitoring without lab equipment.

The paper develops a platform for point-of-care synthesis of peptide hormones using cell-free protein synthesis and affinity purification, enabling local production of therapeutics in resource-limited settings.

This study develops a low-cost recombinant glycoconjugate vaccine against ETEC O148 and O78 that provides immunogenicity and protection in mice.

This paper develops a dynamic kinetic model of cell-free metabolism to optimize butanol production, identifying aldehyde/alcohol dehydrogenase as a key bottleneck.

This paper presents a cell-free expression method for multivalent rhamnose-binding lectins and demonstrates their direct analysis using biolayer interferometry for binding affinity and specificity.

This study uses community science and experimental screening to design mutant ribosomes with improved protein synthesis and cell growth in vitro and vivo.

This paper improves cell-free expression of membrane proteins by identifying factors affecting synthesis and developing a model to optimize yield through membrane composition and N-terminal domain sequence adjustments.