Ashty S. Karim

This paper develops a cell-free biosynthesis system for 1,2,4-butanetriol, achieving high productivity and yield at scale while reducing costs.

The paper uses machine learning to guide cell-free expression of carbonic anhydrases, identifying variants with improved stability under high temperatures.

This paper optimizes low-cost reagent formulations for cell-free gene expression, achieving high yields and cost reductions across multiple scales and protein types.

The paper develops a machine learning-guided workflow to optimize cell-free biosensors for detecting lead in drinking water, achieving sensitivity at the EPA action level and improved selectivity.

This paper presents a cell-free manufacturing platform for producing conjugate vaccines with high glycosylation efficiency and purity, validated through immunogenicity testing in mice.

This paper demonstrates scalable cell-free production of active T7 RNA polymerase with high purity and activity, suitable for decentralized biotherapeutic manufacturing.

This paper develops a semi-automated method for manufacturing cell-free biosensors using liquid handling robotics, demonstrating comparable performance to manual methods.

The paper develops a machine learning-guided cell-free platform to accelerate enzyme engineering by mapping sequence-function relationships and optimizing enzymes for multiple chemical reactions.

This study presents a cell-free biocatalytic system for converting one-carbon substrates into acetyl-CoA using a synthetic pathway called ReForm.

This paper develops a cell-free platform combining CFPS and AlphaLISA to rapidly study and engineer PTM installing proteins for therapeutic applications.

The paper develops four cell-free biosensing modules for synthetic biology education that enable students to explore transcriptional regulation and environmental monitoring without lab equipment.

The paper develops a platform for point-of-care synthesis of peptide hormones using cell-free protein synthesis and affinity purification, enabling local production of therapeutics in resource-limited settings.

This paper develops a dynamic kinetic model of cell-free metabolism to optimize butanol production, identifying aldehyde/alcohol dehydrogenase as a key bottleneck.

This paper develops a low-cost, thermostable cell-free protein synthesis system for producing conjugate vaccines without refrigeration, achieving ~$0.50 per dose after 4 weeks at room temperature.

The paper designs and selects ribosomal active site mutants with high activity using a computational approach to overcome limitations in rRNA modification.

The paper optimizes and implements reverse beta-oxidation pathways in bacteria for carbon-negative synthesis of C4-C6 acids and alcohols, achieving high product titers in engineered strains.

The paper develops a freeze-dried CRISPR-Cas12 sensor for detecting Wolbachia in arthropod samples, suitable for secondary education.

This paper improves genomically recoded E. coli strains for producing proteins with non-canonical amino acids by optimizing nuclease and protease function and introducing an inducible T7 RNA polymerase cassette.

Spacer2PAM predicts functional PAM sequences for endogenous CRISPR systems, enabling targeted genome modification in difficult-to-transform organisms.

The paper introduces a cell-free method called iPROBE to rapidly prototype and optimize biosynthetic pathways for producing chemicals like limonene, increasing production from 0.2 to 4.5 mM in 24 hours.

This paper develops a cell-free system for styrene biosynthesis, achieving titers 10 times higher than cellular methods by optimizing reaction conditions.

The paper introduces iPROBE, a platform for in vitro prototyping and rapid optimization of biosynthetic enzymes, demonstrating improved production of 3-hydroxybutyrate and n-butanol in microbial cell factories.

This paper develops modular plasmids for cell-free expression and genetic assembly in Clostridium to accelerate biosynthetic pathway testing.