Efficient plasmid-based rescue of T7 RNA polymerase-driven calicivirus reverse genetics systems in mammalian cells using vaccinia virus RNA capping enzymes
Frazer J. T. Buchanan, Markella Loi, Charlotte Chim, ShuXian Zhou, Rebekah Penrice-Randal, Leandro Xavier Neves, Maximilian Erdmann, Edward Emmott
This paper introduces a plasmid-based system using vaccinia virus capping enzymes to enhance calicivirus reverse genetics in mammalian cells, significantly increasing viral titres and polyprotein abundance.
Viruses · May 4, 2026Peer-reviewedMicrobiology