MicrobiologyVirusesPeer-reviewed
Efficient plasmid-based rescue of T7 RNA polymerase-driven calicivirus reverse genetics systems in mammalian cells using vaccinia virus RNA capping enzymes
Frazer J. T. Buchanan, Markella Loi, Charlotte Chim…·Edward Emmott
This paper introduces a plasmid-based system using vaccinia virus capping enzymes to enhance calicivirus reverse genetics in mammalian cells, significantly increasing viral titres and polyprotein abundance.
DOI 10.3390/v18050536