Edward Emmott

Viral protease-mediated polyprotein processing in human astroviruses

This study identifies cleavage sites and processing dynamics of astrovirus polyproteins, revealing conserved and divergent features between classical and non-classical strains.

Efficient plasmid-based rescue of T7 RNA polymerase-driven calicivirus reverse genetics systems in mammalian cells using vaccinia virus RNA capping enzymes

This paper introduces a plasmid-based system using vaccinia virus capping enzymes to enhance calicivirus reverse genetics in mammalian cells, significantly increasing viral titres and polyprotein abundance.

Mitophagy-related prohibitin 2 is an orthoflavivirus restriction factor targeted for degradation during infection

This paper identifies prohibitin 2 (PHB2) as an orthoflavivirus restriction factor that is targeted for degradation during infection to enhance viral replication.

Single-cell phenotypic analysis and multiplet detection through incorporation of microscopy data into cellenONE-based single-cell proteomic data analysis

This paper introduces scpImaging, a computational pipeline that uses microscopy data to detect multiplets and improve quality control in single-cell proteomics.

IFIT3 controls IFIT1 accumulation and specificity preventing self mRNA targeting during the innate immune response.

The paper shows IFIT3 regulates IFIT1 stability and activity, preventing self mRNA targeting while maintaining antiviral function during the innate immune response.

Encephalomyocarditis virus protein 2B* antagonises innate immune signalling by interacting with 14-3-3 protein family members

This paper identifies 2B* as an EMCV protein that antagonizes innate immune signaling by interacting with 14-3-3 proteins, specifically through the RRNSS sequence.

The K18-hACE2 mouse model of SARS-CoV-2 infection to illustrate the role and response of the vasculature in neurotropic viral infection

This study uses a K18-hACE2 mouse model to show SARS-CoV-2 infects neurons in the brain without damaging blood vessels, leading to limited neuroinflammation.

Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity

This paper develops SCoPE2, a single-cell proteomic method, to analyze macrophage heterogeneity and finds a continuous proteome gradient in macrophages without polarizing cytokines.

Automated sample preparation for high-throughput single-cell proteomics

Minimal ProteOmic sample Preparation (mPOP) eliminates cleanup losses in single-cell mass spectrometry proteomics, enabling automated SCoPE-MS profiling that separates mammalian cells by cell type and cell-division-cycle phase.