Seven mutations unlock strict synthetic methylotrophy in engineered Pseudomonas putida
Òscar Puiggené, Martina Fricano, Riccardo Rossi, Luc F. M. Jansen, Emre Özdemir, Se Hyeuk Kim, Christina Lenhard, Elsayed T. Mohamed, Stefano Donati, Jochen Förster, Vijayalakshmi Kandasamy, Adam M. Feist, Enrico Orsi, Pablo I. Nikel
The paper engineers Pseudomonas putida to strictly metabolize methanol through seven mutations, enabling growth on methanol as the sole carbon source.
bioRxiv · Jul 22, 2026 · PreprintSynthetic Biology