Enrico Orsi

Adaptive laboratory evolution rewires Pseudomonas putida for resource-efficient acetate assimilation

This study improves acetate tolerance in Pseudomonas putida through adaptive laboratory evolution and identifies gacA and fabB as key targets for efficient acetate assimilation.

Seven mutations unlock strict synthetic methylotrophy in engineered Pseudomonas putida

The paper engineers Pseudomonas putida to strictly metabolize methanol through seven mutations, enabling growth on methanol as the sole carbon source.

A microbial growth-coupled platform for in vivo interrogation of Rubisco oxygenase activity

This paper develops a microbial platform to study Rubisco oxygenase activity in vivo by linking it to bacterial growth, enabling genetic screening and evolution of enzyme variants.

Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system

This study optimizes a genome editing system for Cupriavidus necator H16, achieving high editing efficiency using an improved SIBR-Cas system.

Expanding the biotechnological scope of metabolic sensors through computation-aided designs

This paper presents a computational workflow for designing and testing E. coli metabolic sensors that detect glyoxylate and glycolate across a wide concentration range, with applications in bioengineering and environmental monitoring.

Engineering the biological conversion of formate into crotonate in Cupriavidus necator

This paper engineers the bacterium C. necator to convert formate into crotonate, achieving higher titer, productivity, and yield through metabolic pathway optimization.

Engineered synthetic one-carbon fixation exceeds yield of the Calvin Cycle

The paper engineers a synthetic one-carbon fixation pathway in Cupriavidus necator that achieves higher biomass yield than the native Calvin Cycle.