Tumor-versus-non-tumor T cell enrichment supported by single-cell and spatial phenotyping enables antigen-agnostic prioritization of candidate tumor-reactive TCRs
Volkhard Seitz, Jannik Franzen, Kristin Gennermann, Burkhard Hirsch, Sigrid Schaper, Anja Dröge, Mathias Dreger, Nadine Genzel, Stephanie Plaßmann, Dominik Bents, Jörn Glökler, Thomas Conrad, Christioph Loddenkemper, Karin Schmelz, Markus Morkel, , Sefer Elezkurtaj
The paper identifies tumor-reactive TCRs using T/N ratios and validates them with single-cell and spatial data, showing their tumor association and effector phenotype.
bioRxiv · Sep 24, 2026 · PreprintImmunology