PERRC: Protease Engineering with Reactant Residence Time Control
Sage E Nelson, Jokent T. Gaza, Seyednima Ajayebi, Ron Masse, Raymond Pho, Cianna Scutero, Samantha G. Martinusen, Lawton F. Long, Amor A. Menezes, Alberto Perez, Carl A. Denard
The paper introduces PERRC, a platform for engineering protease specificity by controlling reactant residence time, resulting in a protease variant with high selectivity for a closely related substrate.
ACS Synthetic Biology · May 19, 2025Peer-reviewedSynthetic Biology