Cell-free prototyping of limonene biosynthesis using cell-free protein synthesis
Quentin M. Dudley, Ashty S. Karim, Connor J. Nash, Michael C. Jewett
The paper introduces a cell-free method called iPROBE to rapidly prototype and optimize biosynthetic pathways for producing chemicals like limonene, increasing production from 0.2 to 4.5 mM in 24 hours.
Metabolic Engineering · Sep 1, 2020Peer-reviewedSynthetic Biology